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Bethyl ige
Ige, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+human+set/Human+IgE+ELISA+Quantitation+Set/pm40444920-131-17-20
Average 93 stars, based on 22 article reviews
ige - by Bioz Stars, 2026-09
93/100 stars

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Related Articles

Produced:

Article Title: The adenomatous polyposis coli protein contributes to normal compaction of mitotic chromatin.
Article Snippet: .. To detect Xenopus Set-a we used an anti-human Set (nuclear oncogen, TAF-Ib) antibody (Bethyl Laboratories) produced against residues 175–220 of human Set, which are identical in Xenopus Set-a. ..

Article Title: The Adenomatous Polyposis Coli Protein Contributes to Normal Compaction of Mitotic Chromatin
Article Snippet: .. To detect Xenopus Set-a we used an anti-human Set (nuclear oncogen, TAF-Iβ) antibody (Bethyl Laboratories) produced against residues 175–220 of human Set, which are identical in Xenopus Set-a. ..

Western Blot:

Article Title: The adenomatous polyposis coli protein contributes to normal compaction of mitotic chromatin.
Article Snippet: .. Western Blotting CSF or ULSS extract (1 ml per line) or Hela cell lysed in MEBC buffer (50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 5 mM EGTA, PLoS ONE | www.plosone.org 11 June 2012 | Volume 7 | Issue 6 | e38102 5 mM EDTA, 0.5% NP-40, and 40 mM b-glycerol phosphate) were separated on 4–12% SDS PAGE (Invitrogen), transferred to Protran nitrocellulose membrane (Schleicher & Schuell) and immunoblotted with the following primary antibodies: anti-APCII anti-serum [51] (1:1000), DM1A (Sigma; 1:2000), rabbit polyclonal anti- Xenopus Topo II antbodies (1:1000), sheep polyclonal anti-Xenopus Smc2 ([55], 1:500), anti-human Rbbp7 (Sigma, 1:500) and anti-human SET (nuclear oncogene) (Bethyl Laboratories, 1:500). .. Secondary antibodies used were either horseradish peroxidase-labelled anti-rabbit IgG that were detected using ECL kit (Pierce), or IRDye800/700-conjugated secondary antibodies (Rockland, 1:5000), detected with LiCor Odyssey Imager (Bioscience).

Clinical Proteomics:

Article Title: The adenomatous polyposis coli protein contributes to normal compaction of mitotic chromatin.
Article Snippet: .. Western Blotting CSF or ULSS extract (1 ml per line) or Hela cell lysed in MEBC buffer (50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 5 mM EGTA, PLoS ONE | www.plosone.org 11 June 2012 | Volume 7 | Issue 6 | e38102 5 mM EDTA, 0.5% NP-40, and 40 mM b-glycerol phosphate) were separated on 4–12% SDS PAGE (Invitrogen), transferred to Protran nitrocellulose membrane (Schleicher & Schuell) and immunoblotted with the following primary antibodies: anti-APCII anti-serum [51] (1:1000), DM1A (Sigma; 1:2000), rabbit polyclonal anti- Xenopus Topo II antbodies (1:1000), sheep polyclonal anti-Xenopus Smc2 ([55], 1:500), anti-human Rbbp7 (Sigma, 1:500) and anti-human SET (nuclear oncogene) (Bethyl Laboratories, 1:500). .. Secondary antibodies used were either horseradish peroxidase-labelled anti-rabbit IgG that were detected using ECL kit (Pierce), or IRDye800/700-conjugated secondary antibodies (Rockland, 1:5000), detected with LiCor Odyssey Imager (Bioscience).

Article Title: The Adenomatous Polyposis Coli Protein Contributes to Normal Compaction of Mitotic Chromatin
Article Snippet: .. CSF or ULSS extract (1 μl per line) or Hela cell lysed in MEBC buffer (50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 5 mM EGTA, 5 mM EDTA, 0.5% NP-40, and 40 mM β-glycerol phosphate) were separated on 4–12% SDS PAGE (Invitrogen), transferred to Protran nitrocellulose membrane (Schleicher & Schuell) and immunoblotted with the following primary antibodies: anti-APCII anti-serum (1∶1000), DM1A (Sigma; 1∶2000), rabbit polyclonal anti- Xenopus Topo II antbodies (1∶1000), sheep polyclonal anti- Xenopus Smc2 ( , 1∶500), anti-human Rbbp7 (Sigma, 1∶500) and anti-human SET (nuclear oncogene) (Bethyl Laboratories, 1∶500). .. Secondary antibodies used were either horseradish peroxidase-labelled anti-rabbit IgG that were detected using ECL kit (Pierce), or IRDye800/700-conjugated secondary antibodies (Rockland, 1∶5000), detected with LiCor Odyssey Imager (Bioscience).

SDS Page:

Article Title: The adenomatous polyposis coli protein contributes to normal compaction of mitotic chromatin.
Article Snippet: .. Western Blotting CSF or ULSS extract (1 ml per line) or Hela cell lysed in MEBC buffer (50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 5 mM EGTA, PLoS ONE | www.plosone.org 11 June 2012 | Volume 7 | Issue 6 | e38102 5 mM EDTA, 0.5% NP-40, and 40 mM b-glycerol phosphate) were separated on 4–12% SDS PAGE (Invitrogen), transferred to Protran nitrocellulose membrane (Schleicher & Schuell) and immunoblotted with the following primary antibodies: anti-APCII anti-serum [51] (1:1000), DM1A (Sigma; 1:2000), rabbit polyclonal anti- Xenopus Topo II antbodies (1:1000), sheep polyclonal anti-Xenopus Smc2 ([55], 1:500), anti-human Rbbp7 (Sigma, 1:500) and anti-human SET (nuclear oncogene) (Bethyl Laboratories, 1:500). .. Secondary antibodies used were either horseradish peroxidase-labelled anti-rabbit IgG that were detected using ECL kit (Pierce), or IRDye800/700-conjugated secondary antibodies (Rockland, 1:5000), detected with LiCor Odyssey Imager (Bioscience).

Article Title: The Adenomatous Polyposis Coli Protein Contributes to Normal Compaction of Mitotic Chromatin
Article Snippet: .. CSF or ULSS extract (1 μl per line) or Hela cell lysed in MEBC buffer (50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 5 mM EGTA, 5 mM EDTA, 0.5% NP-40, and 40 mM β-glycerol phosphate) were separated on 4–12% SDS PAGE (Invitrogen), transferred to Protran nitrocellulose membrane (Schleicher & Schuell) and immunoblotted with the following primary antibodies: anti-APCII anti-serum (1∶1000), DM1A (Sigma; 1∶2000), rabbit polyclonal anti- Xenopus Topo II antbodies (1∶1000), sheep polyclonal anti- Xenopus Smc2 ( , 1∶500), anti-human Rbbp7 (Sigma, 1∶500) and anti-human SET (nuclear oncogene) (Bethyl Laboratories, 1∶500). .. Secondary antibodies used were either horseradish peroxidase-labelled anti-rabbit IgG that were detected using ECL kit (Pierce), or IRDye800/700-conjugated secondary antibodies (Rockland, 1∶5000), detected with LiCor Odyssey Imager (Bioscience).

Membrane:

Article Title: The adenomatous polyposis coli protein contributes to normal compaction of mitotic chromatin.
Article Snippet: .. Western Blotting CSF or ULSS extract (1 ml per line) or Hela cell lysed in MEBC buffer (50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 5 mM EGTA, PLoS ONE | www.plosone.org 11 June 2012 | Volume 7 | Issue 6 | e38102 5 mM EDTA, 0.5% NP-40, and 40 mM b-glycerol phosphate) were separated on 4–12% SDS PAGE (Invitrogen), transferred to Protran nitrocellulose membrane (Schleicher & Schuell) and immunoblotted with the following primary antibodies: anti-APCII anti-serum [51] (1:1000), DM1A (Sigma; 1:2000), rabbit polyclonal anti- Xenopus Topo II antbodies (1:1000), sheep polyclonal anti-Xenopus Smc2 ([55], 1:500), anti-human Rbbp7 (Sigma, 1:500) and anti-human SET (nuclear oncogene) (Bethyl Laboratories, 1:500). .. Secondary antibodies used were either horseradish peroxidase-labelled anti-rabbit IgG that were detected using ECL kit (Pierce), or IRDye800/700-conjugated secondary antibodies (Rockland, 1:5000), detected with LiCor Odyssey Imager (Bioscience).

Article Title: The Adenomatous Polyposis Coli Protein Contributes to Normal Compaction of Mitotic Chromatin
Article Snippet: .. CSF or ULSS extract (1 μl per line) or Hela cell lysed in MEBC buffer (50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 5 mM EGTA, 5 mM EDTA, 0.5% NP-40, and 40 mM β-glycerol phosphate) were separated on 4–12% SDS PAGE (Invitrogen), transferred to Protran nitrocellulose membrane (Schleicher & Schuell) and immunoblotted with the following primary antibodies: anti-APCII anti-serum (1∶1000), DM1A (Sigma; 1∶2000), rabbit polyclonal anti- Xenopus Topo II antbodies (1∶1000), sheep polyclonal anti- Xenopus Smc2 ( , 1∶500), anti-human Rbbp7 (Sigma, 1∶500) and anti-human SET (nuclear oncogene) (Bethyl Laboratories, 1∶500). .. Secondary antibodies used were either horseradish peroxidase-labelled anti-rabbit IgG that were detected using ECL kit (Pierce), or IRDye800/700-conjugated secondary antibodies (Rockland, 1∶5000), detected with LiCor Odyssey Imager (Bioscience).



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Image Search Results


Baseline demographic and clinical characteristics according to periodontal stage.

Journal: Biomedicines

Article Title: Fatty Acid Binding Protein 4 Could Be a Linking Biomarker Between Periodontitis and Systemic Diseases

doi: 10.3390/biomedicines13020402

Figure Lengend Snippet: Baseline demographic and clinical characteristics according to periodontal stage.

Article Snippet: The blood sample was centrifuged in the laboratory to separate the serum, and then confirmed the P. gingivalis antibody titer by P. gingivalis antibody titer ELISA kit (Boster Biological Technology, New York, NY, USA) and FABP4 levels using Human FABP4/A Fabp ELISA Kit (PicoKine ® , Pleasanton, CA, USA).

Techniques:

(A) Comparison of indicators before and after non-surgical periodontal treatment. (B) Comparison of indicators before and after periodontal treatment, catagorized by stage.

Journal: Biomedicines

Article Title: Fatty Acid Binding Protein 4 Could Be a Linking Biomarker Between Periodontitis and Systemic Diseases

doi: 10.3390/biomedicines13020402

Figure Lengend Snippet: (A) Comparison of indicators before and after non-surgical periodontal treatment. (B) Comparison of indicators before and after periodontal treatment, catagorized by stage.

Article Snippet: The blood sample was centrifuged in the laboratory to separate the serum, and then confirmed the P. gingivalis antibody titer by P. gingivalis antibody titer ELISA kit (Boster Biological Technology, New York, NY, USA) and FABP4 levels using Human FABP4/A Fabp ELISA Kit (PicoKine ® , Pleasanton, CA, USA).

Techniques: Comparison

( A – C ) FABP4 changes after periodontal treatment in each stage. ( D – F ) P. gingivalis antibody titer level changes after periodontal treatment in each stage.

Journal: Biomedicines

Article Title: Fatty Acid Binding Protein 4 Could Be a Linking Biomarker Between Periodontitis and Systemic Diseases

doi: 10.3390/biomedicines13020402

Figure Lengend Snippet: ( A – C ) FABP4 changes after periodontal treatment in each stage. ( D – F ) P. gingivalis antibody titer level changes after periodontal treatment in each stage.

Article Snippet: The blood sample was centrifuged in the laboratory to separate the serum, and then confirmed the P. gingivalis antibody titer by P. gingivalis antibody titer ELISA kit (Boster Biological Technology, New York, NY, USA) and FABP4 levels using Human FABP4/A Fabp ELISA Kit (PicoKine ® , Pleasanton, CA, USA).

Techniques:

( A – D ) Correlations between changes in FABP4 and Clinical parameters. ( E ) Correlations between changes in FABP4 and P. gingivalis antibody titer.

Journal: Biomedicines

Article Title: Fatty Acid Binding Protein 4 Could Be a Linking Biomarker Between Periodontitis and Systemic Diseases

doi: 10.3390/biomedicines13020402

Figure Lengend Snippet: ( A – D ) Correlations between changes in FABP4 and Clinical parameters. ( E ) Correlations between changes in FABP4 and P. gingivalis antibody titer.

Article Snippet: The blood sample was centrifuged in the laboratory to separate the serum, and then confirmed the P. gingivalis antibody titer by P. gingivalis antibody titer ELISA kit (Boster Biological Technology, New York, NY, USA) and FABP4 levels using Human FABP4/A Fabp ELISA Kit (PicoKine ® , Pleasanton, CA, USA).

Techniques:

Composition of CD8 pos T cells with CD8 pos CD127 low pos/neg FoxP3 pos Tregs and CD8 pos CD127 pos FoxP3 neg Tresps in KTR with stable allograft function (n = 134), KTR with biopsy-proven rejection at enrolment (n = 41), and KTR who developed rejection during the follow-up period (n = 5) and its influence on their Treg/Tresp ratio and the percentage of total CD8 pos T cells. The diagrams exhibit the proportion of Tregs (A) and Tresps (B) within total CD8 pos T cells (cell count of 100.000 for each patient), the CD8 pos Treg/Tresp ratio (C) , and the percentage of total CD8 pos T cells of all lymphocytes (D) of KTR with stable allograft function in light blue (♦), KTR with biopsy-proven rejection at enrolment in red (♦), and KTR who developed rejection during the follow-up period in dark blue (♦). Color-matched regression lines indicate changes with age, with significant changes indicated by the p-value next to the regression line. Age-independent significant differences of KTR with rejection at enrolment or KTR who develop rejection during the follow-up period compared to stable KTR are marked by an arrow and their color-matched P -values.

Journal: Frontiers in Immunology

Article Title: Exhaustion of CD8 pos central memory regulatory T cell differentiation is involved in renal allograft rejection

doi: 10.3389/fimmu.2025.1532086

Figure Lengend Snippet: Composition of CD8 pos T cells with CD8 pos CD127 low pos/neg FoxP3 pos Tregs and CD8 pos CD127 pos FoxP3 neg Tresps in KTR with stable allograft function (n = 134), KTR with biopsy-proven rejection at enrolment (n = 41), and KTR who developed rejection during the follow-up period (n = 5) and its influence on their Treg/Tresp ratio and the percentage of total CD8 pos T cells. The diagrams exhibit the proportion of Tregs (A) and Tresps (B) within total CD8 pos T cells (cell count of 100.000 for each patient), the CD8 pos Treg/Tresp ratio (C) , and the percentage of total CD8 pos T cells of all lymphocytes (D) of KTR with stable allograft function in light blue (♦), KTR with biopsy-proven rejection at enrolment in red (♦), and KTR who developed rejection during the follow-up period in dark blue (♦). Color-matched regression lines indicate changes with age, with significant changes indicated by the p-value next to the regression line. Age-independent significant differences of KTR with rejection at enrolment or KTR who develop rejection during the follow-up period compared to stable KTR are marked by an arrow and their color-matched P -values.

Article Snippet: Intracellular FoxP3 was detected using an anti-human FoxP3 staining set (clone PCH101, eBioscience) according to the manufacturer’s instructions.

Techniques: Cell Counting

Characterization of CD8 pos FoxP3 pos CD127 low pos/neg Tregs as CD8 pos CD25 pos CD127 low pos/neg Tregs and CD8 pos FoxP3 pos CD25 pos Tregs. First, all lymphocytes were detected by FSC-H versus FSC-A (A) and subsequent FSC-A versus SSC-A (B) gating. Then, we examined the fluorescence activity of CD8 versus CD45RA to detect CD8 pos T cells within all lymphocytes (C) . Fluorescence activity of FoxP3 versus CD127 was presented to separate CD8 pos Tregs from Tresps. CD8 pos Tregs were gated as CD8 + FoxP3 + CD127 low pos/neg Tregs within total CD8 pos T cells (0.5%) (D) and presented as CD8 pos CD25 pos CD127 low pos/neg Tregs (97% overlap) (E) and CD8 pos FoxP3 pos CD25 pos Tregs (100% overlap) (F) by analyzing fluorescence activity of CD25 versus CD127 and FoxP3 versus CD25.

Journal: Frontiers in Immunology

Article Title: Exhaustion of CD8 pos central memory regulatory T cell differentiation is involved in renal allograft rejection

doi: 10.3389/fimmu.2025.1532086

Figure Lengend Snippet: Characterization of CD8 pos FoxP3 pos CD127 low pos/neg Tregs as CD8 pos CD25 pos CD127 low pos/neg Tregs and CD8 pos FoxP3 pos CD25 pos Tregs. First, all lymphocytes were detected by FSC-H versus FSC-A (A) and subsequent FSC-A versus SSC-A (B) gating. Then, we examined the fluorescence activity of CD8 versus CD45RA to detect CD8 pos T cells within all lymphocytes (C) . Fluorescence activity of FoxP3 versus CD127 was presented to separate CD8 pos Tregs from Tresps. CD8 pos Tregs were gated as CD8 + FoxP3 + CD127 low pos/neg Tregs within total CD8 pos T cells (0.5%) (D) and presented as CD8 pos CD25 pos CD127 low pos/neg Tregs (97% overlap) (E) and CD8 pos FoxP3 pos CD25 pos Tregs (100% overlap) (F) by analyzing fluorescence activity of CD25 versus CD127 and FoxP3 versus CD25.

Article Snippet: Intracellular FoxP3 was detected using an anti-human FoxP3 staining set (clone PCH101, eBioscience) according to the manufacturer’s instructions.

Techniques: Fluorescence, Activity Assay